Description
Nanoparticle Size
The Eclipse™ DualTec™ system offers both Hollow-Fiber Flow-FFF (HF5) and Asymmetric Flow-FFF (AF4) techniques. Both may be integrated into one instrument and coupled seamlessly to the most advanced light scattering detector for determination of molar mass and nanoparticle size, Wyatt’s DAWN® instrument.
Macromolecules: Complement SEC-MALS
Eclipse FFF systems add to, and complements, size exclusion chromatography (SEC) for macromolecular separations. Separation occurs without shear or adverse, non-ideal column interactions. The Eclipse DualTec allows you to take full advantage of your favorite conventional SEC and AF4/HF5 separations in one convenient package.
The DualTec lets you use the same HPLC pump and autosampler, and the same MALS and DLS light scattering detectors, to characterize macromolecules for molecular weight, size and conformation by SEC or FFF. Switch between the two separation techniques at the click of a mouse.
Nanoparticles: High-resolution characterization
Common solution-based methods for determining the size and size distributions of nanoparticles, such as batch dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA), cannot offer the resolution, statistical confidence and absolute accuracy of a true separation-based technique that incorporates MALS and DLS downstream detection.
FFF-MALS and FFF-DLS performed with an Eclipse DualTec, DAWN and an embedded WyattQELS DLS detection module offer excellent resolution and accuracy in analysis of size distributions. Particle counts and sizes of viruses and virus-like particles estimated by FFF-MALS match closely TEM-based counts.
Key benefits of FFF with a Wyatt Eclipse
- Robust separation of macromolecules and nanoparticles from 1 nm – 1000 nm with excellent resolution
- Typical analysis times 5 to 30 minutes
- No stationary phase – open channel separation with minimal shear and surface interactions
- Versatile choice of separation channels, permitting sample loads from nL to mL, including semi-preparative FFF
- Optimize separation methods to enhance resolution or size range simply by adjusting flow ratios, even programmatically during a run
- Fractions can be collected and used for off-line analysis (with electron microscopy, MS, ICP-MS, ELISA, etc.)
Eclipse DualTec
- Choose between any two of SEC, AF4 or HF5 mode separations with a single HPLC and detector set
- Make use of the highest quality HPLC pumps and autosamplers from Agilent, Shimadzu, Thermo, or Waters
- Integrate with your favorite HPLC software from Agilent, Dionex or Shimadzu
- Couple to ICP-MS for elemental analysis of nanoparticles per size bin
Innovation, innovation, innovation
The innovative Eclipse DualTec makes available two different lines of separation in a single instrument configuration. Each line draws sample from the same autosampler, propelled by the same pump, and characterized by the same detectors. Each separation line can be configured for SEC, AF4 or HF5, independently, so you can:
- Develop a method with tiny sample volumes by HF5, then transfer to AF4 to increase information content*
- Attach two HF5 channels to double the time between replacing cartridges
- Innovate your research with new analytical capabilities
*Method transfer is completed without additional experimental work by applying the SCOUT DPS™ software, a patented procedure to predict and optimize your separation based on the flow rates and channel geometries. SCOUT DPS is sold separately.
