The scattering coefficient of proteins depends on molecular weight and the shape of the protein molecules. Although scatterng coefficient of particles can be determined by various methods, dynamic light scattering method determines scattering coefficient of particles in solution most easily and enables calculation of hydrodynamic radius using the Stokes-Einstein equation. Molecular weight can also be estimated based on given scattering coefficient.
We determined the scattering coefficient of substances (lysozyme, trypsin inhibitor, anhydrase, ovalbumin, and bovine serum albumin) of molecular weights ranging from 10,000 to 70,000 and obtained correlations with molecular weights.
