Liposomes, which have drawn attention as drug carriers for chemotherapy and other therapies, have a bilayer, much like cell membranes in vivo. Specific properties can be conferred on liposomes by enclosing drugs in the bilayer or by adding charged substances and polymers to the surface. In recent years, measurements of the fixed aqueous layer thickness (FALT) of liposomes has been often performed to evaluate drug release and drug effects.
We used liposomes (Liposome KIT L-ɑ-Phosphatidylcholine [egg yolk] for positively charged liposome from Sigma) dispersed in 0.005 to 0.2 M/L aqueous NaCl solution and measured the zeta potential. The liposome concentration was 0.5 to 1 mg/ml.
