Exosome characterization has become a hot topic in molecular biology and medical research. This white paper gives a summary of how the Wyatt Eclipse® Field-Flow Fractionation system coupled to MALS and DLS allows a more detailed and comprehensive characterization of exosomes. Key is a physical separation of exosomes from other components of the biological matrix (serum, urine, etc.) with online determination of size, composition and charge. Fractions can be collected for further off-line analysis, most importantly, sequencing of DNA contained in exosomes. A recent publication by Zhang et.al. in Nature Biology has shown that biological function correlates with size and three distinct size populations could be separated for the first time using the Wyatt Eclipse FFF system. This white paper explains how the separation of exosomes can be achieved and which information is obtained by coupling MALS and DLS online.

null
Download PDF
DAWN®

DAWN®

The world’s most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

Read more
Eclipse AF4

Eclipse AF4

Versatile separations of polymers, proteins, colloids, and nanoparticles.

The Eclipse™ AF4 supports all AF4 techniques except Hollow-Fiber Flow Field-Flow Fractionation (HF5), which is covered by the Eclipse DualTec. However, the Eclipse AF4 supports the fritless AF4 channel which prevents the difficulties encountered by certain samples prone to aggregation at the relatively high concentrations typical of the AF4 focusing step. It also supports the semi-preparative channel.

Read more