Protein Reagent Quality for Drug Discovery

Quality and consistency in reagents is critical to successful drug discovery and development. When targeting a particular protein of interest, in vitro experiments should be performed with proteins of biological properties similar to those for in vivo tests. It is important that molecularity, purity, shape and degree of heterogeneity remain the same when any alterations are made to model protein or the formulation buffer. Multi-angle light scattering (MALS) combined with size exclusion chromatography (SEC-MALS) is a very useful technique to monitor the solution properties of the protein as changes to reagents are made.