One of the primary challenges in developing effective formulations for the nanoscale delivery of therapeutics is particle characterization. Standard techniques such as dynamic light scattering (DLS), particle tracking analysis (PTA) and transmission electron microscopy (TEM) typically suffer from tradeoffs between simplicity, detail and sampling efficiency. For example, DLS is simple to use, and can sample a large particle ensemble, but only provides semi-quantitative, low-resolution size distributions; on the other hand, TEM offers exquisite structural detail, at the cost of complexity, laborious sample preparation and very small ensembles that lead to high statistical uncertainty.

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Eclipse AF4

Eclipse AF4

Versatile separations of polymers, proteins, colloids, and nanoparticles.

The Eclipse™ AF4 supports all AF4 techniques except Hollow-Fiber Flow Field-Flow Fractionation (HF5), which is covered by the Eclipse DualTec. However, the Eclipse AF4 supports the fritless AF4 channel which prevents the difficulties encountered by certain samples prone to aggregation at the relatively high concentrations typical of the AF4 focusing step. It also supports the semi-preparative channel.

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