The analysis of opiates in blood and urine was accomplished using automated Disposable Pipette Extraction (DPX) followed by LC/MS analysis. The automated extractions were performed in about 5 minutes using a GERSTEL MultiPurpose Sampler (MPS). The eluents were subsequently dried and reconstituted in solvent and injected into a HPLC/MS/MS instrument. Recoveries ranged from 60 to 85% for the opiates in blood, and recoveries ranged from 78 to 85% for the opiates in hydrolyzed urine. The %RSDs were lower than 6% for all analytes.

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Disposable Pipette Extraction (DPX)

Disposable Pipette Extraction (DPX)

Extraction in Seconds

DPX is a fast and efficient SPE technique used for a wide rnge of applications such as drugs of abuse, therapeutic drug monitoring, comprehensive screening, pharmacology studies (NNK), as well as pesticides in fruit and vegetables. DPX is based on unique and patented SPE devices: Pipette tips that incorporate loosely contained sorbent material, which is mixed with the sample solution. Turbulent air bubble mixing creates a suspension of sorbent in the sample ensuring optimal contact and highly efficient extraction. The extraction is performed much faster than with traditional SPE techniques.

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