In this work an automated DPX procedure is presented that enables the determination of acidic, basic, and neutral drugs in small blood or urine samples. The DPX method is based on a mixed-mode cation exchange sorbent (CX) with reversed phase characteristics. The automated extraction is shown to require only minimal manual labor when performed using the GERSTEL MPS 2 autosampler under MAESTRO software control. GC-MS data is provided showing that comprehensive, rapid and sensitive analysis can be performed using automated DPX combined with large volume injection. Automated sample preparation steps are performed during the GC/MS run of the preceding sample using the MAESTRO PrepAhead function. PrepAhead sample preparation enables high throughput analysis, improved productivity and “just in time” preparation of each sample.

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Disposable Pipette Extraction (DPX)

Disposable Pipette Extraction (DPX)

Extraction in Seconds

DPX is a fast and efficient SPE technique used for a wide rnge of applications such as drugs of abuse, therapeutic drug monitoring, comprehensive screening, pharmacology studies (NNK), as well as pesticides in fruit and vegetables. DPX is based on unique and patented SPE devices: Pipette tips that incorporate loosely contained sorbent material, which is mixed with the sample solution. Turbulent air bubble mixing creates a suspension of sorbent in the sample ensuring optimal contact and highly efficient extraction. The extraction is performed much faster than with traditional SPE techniques.

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