Chitin is one of the most abundant biopolymers on earth (the other is cellulose). Chitin, or poly-N-acetylglucosamine, is the major polymer in the exoskeleton of marine arthropods and can also be found in fungi and yeasts. It is used in water treatment, photographic emulsions and dyeing improvement of synthetic fibers and fabrics.

Chitosan is deacetylated chitin. It can be obtained from shrimp or crab shells. Its applications vary from the therapeutic, such as wound healing, to cosmetics and dietary supplements.

For many of these applications, it is useful to fully characterize the molar mass moments and distributions of the chitosan products. Size-exclusion chromatography in combination with multi-angle light scattering detection (SEC-MALS) provides an easy method to obtain these properties in an absolute manner, free of molecular references. In this note, we describe the results for two chitosan samples analyzed by SEC-MALS.

DAWN®

DAWN®

The world’s most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

Optilab

Optilab

Universal Detection for Chromatography and FFF Separations

Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the Optilab achieves an unprecedented combination of sensitivity and range. These features mean that it addresses both standard chromatographic applications and some challenges unique to light scattering measurements such as high concentrations, determination of sample refractive increments (dn/dc) and solvent refractive index.