Characterizing key parameters of different heparin lots requires multiple techniques. Weight-average molar mass measured by multi-angle static light scattering (MALS) and hydrodynamic radius measured by dynamic light scattering (DLS) can be used to characterize the size and polydispersity of the molecule. These quantities can also be used for lot-to-lot comparison and to qualify heparin from different suppliers. Massively-parallel phase analysis light scattering (MPPALS) and DLS are used to calculate the net charge and characterize the purity of the sample. In this study, we compared heparin from two suppliers, including one lot modified with super-sulfated material.

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DAWN®

DAWN®

The world’s most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

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Optilab

Optilab

Universal Detection for Chromatography and FFF Separations

Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the Optilab achieves an unprecedented combination of sensitivity and range. These features mean that it addresses both standard chromatographic applications and some challenges unique to light scattering measurements such as high concentrations, determination of sample refractive increments (dn/dc) and solvent refractive index.

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