Liposomes are generally classified as small unilamellar liposomes (small unilamellar vsicle, SUV; 100 nm or less), large unilamellar liposomes (large unilamellar vesicle, LUV; 100 to 500 nm), and multilamellar liposomes (multilamellar vesicle, MLV; 200 to 1000 nm).
We performed particle diameter measurement of these three kinds of liposomes, each prepared by ultrasonic treatment, the reverse phase evaporation method, and the vortex processing method, by dynamic light scattering. Each sample was prepared to a concentration of 5 mM, with a focus on the phosphate part of the phospholipid. To prevent the collapse of the liposome, we removed dust with a polycarbonate filtration membrane with 0.4 µm circular holes.
