Multi-angle light scattering (MALS) is primarily used to determine molecular weight, size- and shape-related parameters of macromolecules or nanoparticles as they elute from a separation device. In batch (unfractionated) mode, MALS provides the weight-average molecular weight <M>w and the z-average mean square radius <Rg 2>z. The DAWN® HELEOS® II is particularly well suited for these tasks owing to its flow cell design, holding a mere ~67 µl and readily connecting to a fluid line through HPLC-type fittings. This very same design can be used profitably to study a sample evolving in time, such as a polymerization or degradation reaction, by injecting a sample into the flow cell with a stopped-flow apparatus.

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DAWN®

DAWN®

The world’s most advanced light scattering instrument for absolute characterization of proteins, conjugates, macromolecules, and nanoparticles.

The DAWN and its companion Optilab dRI detector are the established benchmarks for MALS analysis, cited in thousands of peer-reviewed publications. Multi-angle light scattering detection is indispensable for use with GPC and HPLC-SEC in order to obtain reliable molecular mass distributions and information on molecular conformation, branching ratio, fragments and aggregates.

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