Domain antibodies (dAbs) are the smallest functional binding units of antibodies, corresponding to the variable regions of either the heavy (VH) or light (VL) chains of human antibodies. Different dAbs can show different degrees of self-association which drives the need for a cheap, quick—yet robust—technique to study self-association behavior in drug discovery. This study outlines the use of SEC-MALS to determine self-association properties and validates the work by comparison with Analytical Ultracentrifugation (AUC).

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miniDAWN®

miniDAWN®

The Best in Basic Multi-Angle Light Scattering for Absolute Macromolecular Analysis.

The miniDAWN’s performance is exceeded only by the DAWN which covers a wider range of size and molecular weight, with even better sensitivity and additional optional modules.

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Optilab

Optilab

Universal Detection for Chromatography and FFF Separations

Using a combination of cutting-edge semiconductor photodiode technology and proprietary computer algorithms, the Optilab achieves an unprecedented combination of sensitivity and range. These features mean that it addresses both standard chromatographic applications and some challenges unique to light scattering measurements such as high concentrations, determination of sample refractive increments (dn/dc) and solvent refractive index.

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