Evaluating the zeta potential of proteins at each pH is important in controlling the dispersion/cohesion and adsorption to surfaces of solids such as test containers. Proteins are known to exhibit different isoelectric points depending on the pKa of amino acids forming the proteins.
We used bovine serum albumin (BSA), a protein containing higher ratios of neutral amino acids, and lysozyme, a protein containing higher ratios of basic amino acids, to measure the pH dependence of zeta potential of each.
